HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system.

ABSTRACT: Self-organised three-dimensional (3D) cell cultures, collectively called 3D-oids, include spheroids, organoids and other co-culture models. Systematic evaluation of these models forms a critical new generation of high-content screening (HCS) systems for patient-specific drug analysis and cancer research. However, the standardisation of working with 3D-oids remains challenging and lacks convincing implementation. This study develops and tests HCS-3DX, a next-generation system for HCS analysis in 3D imaging and image evaluation. HCS-3DX is based on three main components: an automated Artificial Intelligence (AI)-driven micromanipulator for 3D-oid selection, an HCS foil multiwell plate for optimised imaging, and image-based AI software for single-cell data analysis. We validated HCS-3DX direc

SUBMITTER: Diosdi A 

PROVIDER: S-EPMC12504613 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature