Optogenetic induction of subcellular Ca²⁺ events in megakaryocytes and platelets using a highly Ca²⁺-conductive channelrhodopsin.

ABSTRACT: Calcium signaling is crucial across various cell types, but its spatiotemporal dynamics remain difficult to study due to limited methods. Optogenetics, with its high precision, can address this challenge. In this study, we introduced the channelrhodopsin variant ChR2 XXM2.0, which exhibits high light sensitivity and enhanced Ca²⁺ conductance in Xenopus oocytes, into bone marrow-derived megakaryocytes through viral transduction, aiming to clarify the poorly understood role of Ca²⁺ dynamics in these cells. ChR2 XXM2.0 expression was confirmed in megakaryocyte membranes, and its functionality validated through whole-cell patch-clamp and calcium imaging. Localized activation of ChR2 XXM2.0 at the cell periphery induced cell polarization, dependent on localized calcium inf

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12504666 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature