Prediction of Secondary Structure Content of Proteins Using Raman Spectroscopy and Self-Organizing Maps.

ABSTRACT: Proteins are biomolecules with characteristic three-dimensional (3D) arrangements that render them different vital functions. In the last 20 years, there has been a growing interest in biopharmaceutical proteins, especially antibodies, due to their therapeutic application^. The functionality of a protein depends on the preservation of its native form, which under certain stressing conditions can undergo changes at different structural levels that cause them to lose their activity.¹ Although mass spectrometry is a powerful technique for primary structure determination, it often fails to give information at higher order levels. Like infrared (IR), Raman spectra are well known to contain bands (especially the amide I from 1625-1725cm^-1) that correlate with seco

SUBMITTER: Pinto Corujo M 

PROVIDER: S-EPMC12504791 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature