Potential of Heparan Sulphate Mimetics Integrated Into Collagen Scaffolds for Enhanced Skin Wound Healing.

ABSTRACT: Optimal healing of full-thickness skin wounds remains a clinical challenge. While current skin substitutes aid burn wound management, there is still a need to effectively minimize scarring. Therefore, we developed type I collagen scaffolds with covalently bound ReGeneraTing Agent (RGTA) OTR4120 (OTR), a synthetic heparan sulphate analogue resistant to glycanase degradation (Col I + OTR). To further stimulate skin regeneration, collagen scaffolds with and without OTR4120 were subsequently loaded with sonic hedgehog (SHH), a key effector molecule in embryogenesis. The presence of OTR4120 and SHH in scaffolds was biochemically and histologically confirmed after crosslinking and sterilization. SHH was found deeper into collagen scaffolds in the presence of OTR4120. Addition of SHH to scaffolds

SUBMITTER: Avila-Martinez N 

PROVIDER: S-EPMC12504924 | biostudies-literature | 2025 Sep-Oct

REPOSITORIES: biostudies-literature