Ultrasonic-assisted acidified aqueous two-phase extraction for one-step production and recovery of flavonoid aglycones from Malvaviscus arboreus Cav. flower: Process exploration, composition analysis and activity validation.

ABSTRACT: Using a polyethylene glycol (PEG)/ammonium sulfate system, a novel approach for one-step extraction and separation of the flavonoid aglycones from Malvaviscus arboreus Cav. flower (MACF) was developed by combining ultrasonic-assisted extraction (UAE) with acidified aqueous two-phase extraction (AATPE). Under the optimized UAE-AATPE conditions of PEG 600 of 22.8 % (w/w), ammonium sulfate of 22.5 % (w/w), 1.5 mol/L HCl, hydrolysis temperature of 85 ℃, and reaction time of 60 min, MACF flavonoid glycosides were not only fully extracted but also completely hydrolyzed, and the produced aglycones were purified by biphasic separation with a yield of 35.90 ± 0.96 mg/g. The mechanism in the UAE-AATPE process including UAE and ultrasonic-assisted acid hydrolysis (UAAH), was explored by investigating

SUBMITTER: Yuan T 

PROVIDER: S-EPMC12504977 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature