DUOX2 activation drives bacterial translocation and subclinical inflammation in IBD-associated dysbiosis.

ABSTRACT:

Background

Inflammatory bowel diseases (IBDs) are characterised by dysbiosis and a leaky gut. The NADPH oxidase dual oxidase 2 (DUOX2) is upregulated in patients with IBD, yet its role in driving the disease remains unclear.

Objective

We interrogated the functional consequences of epithelial DUOX2 activity for the host and microbiome.

Design

DUOX2 function was studied in mice with epithelial-specific DUOX2 overactivation (vTLR4), inactivation (vTLR4 DUOXA IEC-KO) and wild-type controls. We assessed the effect of dysbiosis on DUOX2 signalling and intestinal permeability (FITC-dextran, serum zonulin, bacterial translocation) with germ-free (GF) mice engrafted with IBD or healthy microbiota. RNA sequencing of colonic mucosa and microbiota and faecal metabolomics were use

SUBMITTER: Hazime H 

PROVIDER: S-EPMC12505107 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature