Labeling, isolation and characterization of cell-type-specific exosomes derived from mouse skin tissue.

ABSTRACT: Extracellular vesicles are a heterogeneous group of membrane-bound vesicles involved in cell-cell communication, formed at the plasma membrane (ectosomes) or by endocytosis (exosomes). Most exosome studies so far have focused on in vitro systems or exosomes derived from bodily fluids, while tissue-derived exosomes remain underexplored. Here we present a protocol using cell-type-specific promoter-driven reporter constructs for the targeted labeling and subsequent isolation of exosomes from specific cell types in vivo from mouse tissues. The differentiation between exosomes and ectosomes remains challenging due to limitations of current isolation techniques that are primarily based on size, density or surface markers. To address this issue, our approach leverages genetic engineering to mark

SUBMITTER: Yadav A 

PROVIDER: S-EPMC12505222 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature