Roles of protein-protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctions.

ABSTRACT: Tricellular tight junctions (tTJs) seal the space between three or more cells in epithelial monolayers. These specialized tight junctions have distinct protein components, including a transmembrane protein tricellulin. However, the mechanisms by which tricellulin localizes specifically to tTJs are incompletely understood. We demonstrate that tricellulin undergoes rapid lateral diffusion along bicellular junctions but is a very stable component of tTJs. BioID proteomics identified several proximity partners of tricellulin, and knockout studies on angulin-1/LSR, occludin and afadin provided evidence that these proteins control tricellulin accumulation to tTJs to different extents and mechanisms. Tricellulin localization was disrupted in afadin and angulin-1/LSR knockout cells, although these

SUBMITTER: Mushtaq T 

PROVIDER: S-EPMC12505278 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature