Tumor-infiltrating lymphocytes-derived CD8⁺ clonotypes infiltrate the tumor tissue and mediate tumor regression in glioblastoma.

ABSTRACT: Adoptive cell therapy with tumor-infiltrating lymphocytes (TILs) has demonstrated consistent clinical efficacy in treating advanced melanoma and other "hot" tumors. However, it has shown limited success in "cold" tumors like glioblastoma. We present the successful treatment of a rapidly progressing glioblastoma patient with TILs expanded using a defined cytokine combination of IL-2, IL-15, and IL-21. The patient received lymphodepletion with cyclophosphamide one day pre-TIL infusion, followed by a single dose of IL-2 post-transfer. Complete tumor regression was observed after two TIL infusions administered two weeks apart. The TIL products were enriched for CD8⁺ T-cells and demonstrated specific lysis of the autologous tumor cell line. Transcriptomic analysis of tumor biopsies p

SUBMITTER: Arruda LCM 

PROVIDER: S-EPMC12505509 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature