Reproducible generation of Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assays.

ABSTRACT: Nipah virus (NiV) is a pathogen to be handled in BSL-4 facilities. Multiple surrogate systems such as virus-like particles (VLPs) and pseudoviruses enable carrying out NiV neutralization assays and study of virus entry pathways in biosafety level-2 (BSL-2) facilities. These are dual protein expression and surface display systems comprising NiV structural glycoproteins F and G as the key components. During generation of NiV VLPs or pseudovirions, ensuring batch to batch uniformity is a major concern due to the lack of proportionate incorporation of these proteins in the producer cells as well as their consistent incorporation in the particles. We established HEK293 pseudovirion producer cells that stably co-incorporated NiV F and G proteins to address the issue. Fluorescence-activated cell

SUBMITTER: Varghese GR 

PROVIDER: S-EPMC12506246 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature