Single-cell sequencing reveals distinct peripheral immune responses in anti-MDA5 antibody positive dermatomyositis with rapidly progressive interstitial lung disease.

ABSTRACT:

Background

Anti-melanoma differentiation-associated protein 5 antibody positive dermatomyositis (MDA5⁺ DM) is an autoimmune disease related to rapidly progressive interstitial lung disease (RPILD) with high mortality. However, the pathogenesis of MDA5⁺ DM with RPILD remains unclear. We aimed to explore the peripheral immune landscape of MDA5⁺ DM with RPILD using single-cell RNA sequencing (scRNA-seq).

Methods

We performed scRNA-seq of peripheral blood mononuclear cells (PBMCs) from MDA5⁺ DM with RPILD (n = 4), MDA5⁺ DM with ILD (non-RPILD, n = 3), and healthy controls (HCs, n = 3).

Results

The proportion of CD14⁺ monocytes increased, but the proportion of natural killer cells, CD4⁺ T cells an

SUBMITTER: Weng C 

PROVIDER: S-EPMC12506285 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature