Baicalein inhibits mycobacterium tuberculosis-induced macrophage M1 polarization depending on the regulation of YY1/RAB10/TLR4 pathway.

ABSTRACT:

Background

Baicalein (Bai) has been found to alleviate the progression of tuberculosis (TB) by inhibiting mycobacterium tuberculosis (M.tb)-induced macrophage pyroptosis, so it may be used as an adjuvant treatment for TB. However, the underlying molecular mechanism of Bai remains unclear.

Methods

THP-1 macrophages were infected with M.tb and treated with Bai. The viability and apoptosis of macrophages were examined with CCK8 assay, flow cytometry and TUNEL staining. The levels of inflammatory cytokines were tested by ELISA. Macrophage M1 polarization was assessed by detecting CD86⁺ cell rate using flow cytometry. The protein levels of RAB10, YY1, TLR4, MYD88, p-P65/P65 and p-IκBα/IκBα were determined using western blot. The interaction between RAB10 and YY1 or TLR

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC12506312 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature