Heterologous expression of the hypovirus CHV1-EP713 full-length cDNA in Botrytis cinerea: transformation with Agrobacterium tumefaciens and evaluation of changes in the fungal phenotype.

ABSTRACT:

Background

Botrytis cinerea is a phytopathogenic fungus responsible for gray mold disease in a wide range of hosts, including ornamentals, vegetables, and fruit-bearing plants. Similarly, Cryphonectria parasitica infects the American chestnut, causing a lethal condition known as chestnut blight. From this species, the CHV1-EP713 virus, classified as a hypovirus due to its ability to reduce fungal virulence, has been isolated and characterized. Building on this knowledge, we aimed to express the full-length cDNA of CHV1-EP713 in B. cinerea to asess whether its expression could alter the fungal phenotype.

Results

To achieve the expression of the hypovirus cDNA in B. cinerea, the pXH9 vector encoding the CHV1-EP713 cDNA and the p18 plasmid containing the Agrobacterium tumefacie

SUBMITTER: Cottet L 

PROVIDER: S-EPMC12506336 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature