PARP inhibition augments immunomodulatory function of mesenchymal stem/stromal cells by promoting STAT1 phosphorylation.

ABSTRACT:

Background

Mesenchymal stem/stromal cells (MSCs) have shown significant therapeutic effects in a range of autoimmune and hyperinflammatory diseases through their strong immunomodulatory properties, both in animal models and clinical settings. Exposure to inflammatory cytokines is crucial for MSCs to acquire immunoregulatory functions; however, the detailed mechanisms underlying this process remain largely unexplored.

Method

MSCs with pharmacological inhibition or genetic knockout of poly ADP-ribose polymerase1 (PARP1) were treated with the inflammatory factors IFNγ and TNFα for 24 h. The mRNA and protein expression of chemokines and immunosuppressive molecules were detected by qRT-PCR and Western blotting, respectively. The therapeutic efficacy of MSCs was evaluated using a

SUBMITTER: Wang T 

PROVIDER: S-EPMC12506346 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature