High fat diet exacerbated glycolipid metabolism disorder in kidney Yang deficiency rats by interfering with IRS 1-PI3K (p85) -Akt-GLUT 4 pathway.

ABSTRACT:

Background

Kidney Yang deficiency (KYD) is associated with disturbances in glucose and lipid metabolism. This study examined whether KYD rats on a high-fat diet (HFD) exhibit aggravated metabolic dysfunction and sought to elucidate the underlying mechanisms.

Methods

Three-week-old SD rats were assigned to control (Con), KYD, HFD, and KYD + HFD groups. KYD models were induced through cold exposure and gastric perfusion of Cortex Phellodendri. The HFD groups were provided high-fat diets. After 10 weeks, metabolic parameters, organ weights, blood glucose/lipid profiles, and protein levels (PGC1α, PPARγ, IRS1, Akt, PI3K(p85), GLUT4) were assessed.

Results

KYD rats exhibited lower body temperature, weight, and weight gain (P< 0.01) compared to controls. Liver weight was re

SUBMITTER: Li H 

PROVIDER: S-EPMC12506369 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature