High MGMT expression identifies aggressive colorectal cancer with distinct genomic features and immune evasion properties.

ABSTRACT:

Introduction

The epigenetic silencing of O⁶-methylguanine DNA methyltransferase (MGMT) is associated with reduced DNA repair capacity, carcinogenesis and increased sensitivity to alkylating chemotherapy. However, the biological role and clinical significance of MGMT overexpression in cancer remains poorly understood.

Methods

Using multiplexed quantitative immunofluorescence we measured the localized levels of MGMT protein, γH2AX and CD8+ T cells in multiple retrospective colorectal cancer (CRC) cohorts. Genomic and transcriptomic features of selected cases were also studied with whole exome DNA sequencing and genome-wide methylation analysis. MGMT-methylated human CRC cells SW620 were transfected with an MGMT-containing plasmid and co-cultured

SUBMITTER: Zhang JY 

PROVIDER: S-EPMC12506448 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature