Integration of lymphatic vasculature to a human lymph node-on-chip enhances physiological immune properties.

ABSTRACT: To study systemic human innate and adaptive immune responses in detail, competent in vitro lymph node (LN) models with LN stromal cells (LNSCs) are required to recapitulate the physiological microenvironment. The multicellular organisation of LNs possesses a challenge for designing such microphysiological systems (MPS), particularly with the structural complexity of LNs and the lymphatic vasculature. Here, we established an organotypic LN model with integrated lymphatics in an organ-on-chip (OoC) platform containing a printed sacrificial structure, and studied the influence of a perfused lymphatic endothelial cell (LEC)-lined channel on the LN-on-chip microenvironment. Upon one-week of culture under lymphatic flow, LECs lined the tubular structure forming a lymphatic vessel through

SUBMITTER: Morrison AI 

PROVIDER: S-EPMC12506468 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature