Isolation and immortalization of human portal vein endothelial cells: A novel research tool for studying splanchnic vasculature.

ABSTRACT:

Background & aims

Cirrhosis profoundly impacts extrahepatic vasculature, particularly altering the portal venous system, leading to increased portal pressure, portosystemic collaterals, and portal vein thrombosis, which heightens morbidity and reduces survival in liver disease. Although intrahepatic vascular changes in cirrhosis are well studied, molecular insights into extrahepatic alterations in the splanchnic region remain limited owing to the inaccessibility of the human portal vein and suboptimal preclinical models. Here, we aim to isolate, characterize, and immortalize primary human portal vein endothelial cells (PVECs) to enhance understanding of pathophysiological changes during liver disease and establish a platform for future drug testing.

Methods

PVECs (n = 12) an

SUBMITTER: Anton A 

PROVIDER: S-EPMC12506482 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature