A proteomics investigation of primary human articular chondrocyte isolation.

ABSTRACT:

Objective

Human primary articular chondrocytes (hPACs) are routinely isolated from articular cartilage for pre-clinical OA research. Collagenase digest of tissue is an essential step, yet the impact of lengthy enzymatic incubation on the hPAC phenotype is unclear. We aimed to delineate this through proteomic analysis.

Design

hPACs were isolated from human knee cartilage (n ​= ​4) from patients undergoing total knee replacement. Collagenase treatment was performed with or without prior fixation of the tissue. Proteomes were quantified using LC-MS/MS. The Proteomic Ruler was employed to estimate protein copy numbers and cell protein masses. Significant differences in protein intensities were determined using paired t-testing and Benjamini-Hochberg correction. Proteomic data we

SUBMITTER: Brumwell A 

PROVIDER: S-EPMC12506514 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature