New Andes virus isolate haplotype obtained during prospective close contacts follow-up of an Hantavirus cardiopulmonary syndrome fatal case, Chile.

ABSTRACT: Andes virus (ANDV) is a zoonotic orthohantavirus that causes hantavirus cardiopulmonary syndrome. Transmission occurs mainly through contact with infected rodent excreta and, less frequently, between humans. Viral isolation from human samples is rare; in Chile, only one strain (CHI-7913, 2002) has been reported. The limited number of isolates reflects the challenge of obtaining samples during early viremia before antibodies appear, and maintaining long-term production of infectious particles in culture. We investigated an asymptomatic case from a family cluster, sampled during early infection. The absence of anti-ANDV antibodies in sera was confirmed by ELISA. Blood fractions were used to infect Vero E6 cells for six weeks, with infectivity monitored by RT-qPCR and Immunofluorescence. We o

SUBMITTER: Barrera A 

PROVIDER: S-EPMC12506574 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature