High-Speed Raman Readout of Single Polypeptides via Plasmonic Nanopores.

ABSTRACT: The ability to identify individual protein molecules using Surface-Enhanced Raman Scattering (SERS) spectroscopy, without the need for labelling, is a significant advancement in biomedical diagnostics. However, the inherently small Raman scattering cross-section of most (bio) molecules necessitates significant signal amplification for successful detection, particularly at the single-molecule level. A novel approach is introduced for fabricating plasmonic nanopores suitable for sequential Raman readout, namely the recording of Raman spectra from portions of molecules, which are progressively flowing into plasmonic hot spots. The method is based on Capillary-Assisted Particle Assembly (CAPA)of gold nanoparticles (Au NPs), thus ensuring high stability and cost-effectiveness. By electrophoreti

SUBMITTER: Khozeymeh Sarbishe F 

PROVIDER: S-EPMC12506624 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature