Clinical Impact of Co-pathogens in Children Infected With Influenza C Virus Based on Viral Load in Clinical Specimens.

ABSTRACT:

Background

We have monitored influenza C virus infection using the cell culture method due to its advantage in detecting infectious virus. Application of multiplex polymerase chain reaction (PCR) improves the detection rate of multiple pathogens from the same clinical specimen, and it can be difficult to determine whether the influenza C virus is the cause of the patient's symptoms. This study aimed to evaluate the impact of co-pathogens in children infected with the influenza C virus based on viral load in clinical specimens.

Methods

We enrolled 11 patients in whom influenza C virus and co-pathogens were isolated by cell culture. Pathogens were further detected by multiplex real-time PCR and next-generation sequencing (NGS). Viral load was determined by quantitative real-ti

SUBMITTER: Matsuzaki Y 

PROVIDER: S-EPMC12506693 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature