Programmed Death Ligand 1-Expressing Macrophages and Their Protective Role in the Joint During Arthritis.

ABSTRACT:

Objective

Arthritis associated with immune checkpoint inhibitor therapies highlights the importance of immune checkpoint expression for joint homeostasis. We investigated the role of programmed death ligand (PD-L) 1 in the synovium using a collagen-induced arthritis (CIA) mouse model.

Methods

We blocked PD-L1 using blocking antibodies during CIA and assessed the arthritis severity by clinical and histologic scoring. PD-L1 expression and the origin of synovial macrophages were investigated using flow cytometry and parabiosis. We used Cre-Lox mice to ascertain the protective role of PD-L1-expressing macrophages in arthritis. The immune profile of human and murine synovial PD-L1⁺ macrophages was determined by reverse transcriptase-polymerase chain reaction, flow cyto

SUBMITTER: Wood MK 

PROVIDER: S-EPMC12506893 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature