3D-bioprinted liver cancer models derived from primary hepatocytes for simulating cancer initiation and drug screening.

ABSTRACT:

Background

HCC remains one of the most lethal cancers globally, and accurately replicating the early events of tumor evolution remains a critical challenge.

Methods

In this study, we developed early-stage liver cancer cell lines by introducing distinct combinations of oncogenes into primary mouse hepatocytes. Using 3D bioprinting technology, combined with bioinks composed of gelatin and alginate, we constructed a more precise representation of liver cancer tissue to better simulate key tumor characteristics.

Results

Our·findings revealed that different oncogene combinations produced unique drug response profiles, with Ras-driven cells exhibiting heightened sensitivity to ferroptosis. Furthermore, 3D bioprinting tumor tissues derived from transformed hepatocytes effect

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC12506986 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature