The CsWRKY50-CsREM1-CsTSⅠ module inhibits theanine biosynthesis in tea plants under drought stress.

ABSTRACT: Drought affects theanine biosynthesis in tea (Camellia sinensis L.) plants, but how drought stress affects the associated regulatory mechanisms remains unclear. Here, we explored the molecular regulatory network governing theanine biosynthesis under drought stress. Prolonged drought stress significantly reduced theanine content and the expression of C. sinensis theanine synthase Ⅰ (CsTSⅠ) in tea plant leaves. We employed yeast 1-hybrid (Y1H) screening using the CsTSⅠ promoter as bait to identify transcription factors regulating CsTSⅠ transcription. Analysis of the drought stress transcriptome facilitated identification of the transcription factor C. sinensis REPRODUCTIVE MERISTEM 1 (CsREM1), whose encoding gene had Fragments Per Kilobase of exon model per Million mapped fragments values si

SUBMITTER: Ye S 

PROVIDER: S-EPMC12507091 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature