Streptococcus cristatus reduces cariogenicity of saliva-derived microcosms under pH-dependent conditions.

ABSTRACT:

Background

The study aims to investigate Streptococcus cristatus, an oral commensal bacterium, as a probiotic for dental caries prevention by modulating the oral microbiome.

Methods

Saliva from four healthy donors was used to establish 24-h microcosm biofilms in an in vitro 96-well peg model. The preformed biofilms were then exposed to biofilm medium containing 0.2% sucrose (BM), with or without S. cristatus. They were grown for 48 h under two conditions: a constant pH-neutral regime (BM supplemented with 76 mM K₂HPO₄ and 15 mM KH₂PO₄, pH 7.0) or cariogenic pH-cycling regime (8 h pH-neutral and 16 h in BM containing 100 mM acetic acid, pH 5.5). Phosphate and acetate buffers were used to control pH. After 72 h,

SUBMITTER: Cai Y 

PROVIDER: S-EPMC12507114 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature