Expression, localization and regulation of NADPH oxidases in pancreatic beta cells.

ABSTRACT:

Objectives

Reactive oxygen species (ROS) are short-lived and act in a site-specific manner, underscoring the importance of identifying the subcellular localization of their sources. ROS-generating NADPH oxidases (NOX) regulate pancreatic beta cell (dys)function. However, their subcellular localization and cytokine-mediated regulation in these cells remain largely unknown. We characterized the expression, subcellular localization and time-dependent cytokine-induced regulation of NOX isoforms in beta cells.

Methods

Isoforms were studied via RT-qPCR, immunoblotting and immunofluorescence in rat islets and beta cell lines.

Results

Beta cells express DUOX1 and DUOX2 proteins and Duoxa2 transcripts; lacking Duoxa1 expression. In INS-1E cells, NOX1 and DUOX1 lo

SUBMITTER: de Almeida DC 

PROVIDER: S-EPMC12507117 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature