Establishing disease-specific thresholds for automated hematopoietic progenitor cell counting in hematological malignancies.

ABSTRACT:

Background

Flow cytometry remains the gold-standard method for enumerating CD34 + cells during peripheral blood stem cell (PBSC) transplantation, but it is resource- and time-intensive. This study aimed to assess the correlation between hematopoietic progenitor cell (HPC) counts measured by a Sysmex XN-series analyzer and CD34 + cell counts obtained by flow cytometry in pre-apheresis peripheral blood and apheresis products, and to establish HPC cutoffs for predicting adequate PBSC yields.

Methods

We analyzed 334 samples (167 pre-apheresis peripheral blood, 167 PBSC apheresis products) collected from patients and healthy donors. HPC and CD34 + cell counts were measured in all samples. Receiver operating characteristic (ROC) curve analysis was performed to determine HPC cutoff

SUBMITTER: Taka O 

PROVIDER: S-EPMC12507282 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature