A Prominent Pro-Inflammatory Phenotype Is Observed in Replication and Stress-Induced Senescent Mast Cells.

ABSTRACT: Mast cells (MC) are long-lived important immune effectors that control inflammation, allergies, and innate immunity reactions, but the expression of specific markers in replicative and stress-induced senescence in this cell type, together with its relevance in vivo, has not been described. Here, bone marrow-derived MCs (BMMC) were generated from young C57BL6/J mice and kept in culture for a long time or treated with the well-known stressor bacterial lipopolysaccharide (LPS) to promote replicative and stress-induced senescence, respectively. Changes in size, granularity, and expression of p16^INK4A and p21^CIP1/WAF1, together with cell cycle arrest and senescence-associated-β-Galactosidase (SA-β-Gal) activity, were observed after 12 weeks in culture, with minimal changes

SUBMITTER: Ibarra-Sanchez A 

PROVIDER: S-EPMC12507405 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature