High-content high-resolution microscopy and deep learning-assisted analysis reveals host and bacterial heterogeneity during <i>Shigella</i> infection.

ABSTRACT: Shigella flexneri is a Gram-negative bacterial pathogen and causative agent of bacillary dysentery. S. flexneri is closely related to Escherichia coli but harbours a virulence plasmid that encodes a type III secretion system (T3SS) required for host cell invasion. Widely recognised as a paradigm for research in cellular microbiology, S. flexneri has emerged as important to study mechanisms of cell-autonomous immunity, including septin cage entrapment. Here, we use high-content high-resolution microscopy to monitor the dynamic and heterogeneous S. flexneri infection process by assessing multiple host and bacterial parameters (DNA replication, protein translation, T3SS activity). In the case of infected host cells, we report a reduction in DNA and protein s

SUBMITTER: Lopez-Jimenez AT 

PROVIDER: S-EPMC12507436 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature