Organotypic culture system for prepubertal mice testicular tissue: A comparative study.

ABSTRACT:

Background

Prepubertal testicular organotypic culture serves as an in vitro model for research in regenerative medicine, developmental biology, and toxicology. However, the selection of culture systems for diverse applications and purposes lacks explicit rationale or standardized guidelines.

Objective

To evaluate the developmental status of germ cells and Sertoli cells in prepubertal testicular organotypic cultures using different media and tools, and to provide evidence for model standardization.

Materials and methods

Immature testicular tissues (ITTs) were isolated from prepubertal mice and cultured in combinations of three media (Medium 1/2/3, M1/2/3) and two tools (agarose gel pillars, tissue culture inserts) at 34°C with 5% CO_₂ for 8 days. After

SUBMITTER: Deng L 

PROVIDER: S-EPMC12507631 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature