Quantitative imaging of lipid transport in mammalian cells.

ABSTRACT: Eukaryotic cells produce over 1,000 different lipid species that tune organelle membrane properties, control signalling and store energy^1,2. How lipid species are selectively sorted between organelles to maintain specific membrane identities is largely unclear, owing to the difficulty of imaging lipid transport in cells³. Here we measured the retrograde transport and metabolism of individual lipid species in mammalian cells using time-resolved fluorescence imaging of bifunctional lipid probes in combination with ultra-high-resolution mass spectrometry and mathematical modelling. Quantification of lipid flux between organelles revealed that directional, non-vesicular lipid transport is responsible for fast, species-selective lipid sorting, in contrast to the slow, unsp

SUBMITTER: Iglesias-Artola JM 

PROVIDER: S-EPMC12507682 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature