Hypermethylation of microRNA-34c CpG Island predicts unfavorable prognosis in acute myeloid leukemia.

ABSTRACT: The expression of microRNAs is regulated by CpG island hypermethylation in acute myeloid leukemia (AML). MiR-34c CpG island methylation status and miR-34c expression were tested in 205 de novo AML patients and 51 healthy controls. MiR-34c-mimics were transfected into THP-1 cells lines. Then Cell viability after transfection and apoptosis were detected. Western blot was used to detected the protein expression of p53. We found the down-regulation of miR-34c was associated with hypermethylation of the neighboring CpG island, and decitabine treatment rapidly restored miR-34c expression. Hypermethylation of the miR-34c CpG island was frequently observed in primary AML patients' bone marrow but not in healthy donors'. Hypermethylation of the miR-34c CpG island correlated with shorter overall sur

SUBMITTER: Yang D 

PROVIDER: S-EPMC12508102 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature