The yeast checkpoint kinase Mec1p functions in transcription termination by facilitating recruitment of Pcf11p and regulating the torpedo exonuclease Rat1p.

ABSTRACT: Transcription cycle of RNA polymerase II (RNAPII) features three stages: initiation, elongation, and termination. Termination, closely linked with pre-mRNA 3' processing, dissociates RNAPII from DNA and releases the nascent RNA transcript. Efficient termination is required for maintaining a pool of RNAPII that is available for re-entry into new transcription cycle. Previous results showed that inactivation of checkpoint kinase Mec1p in the absence of exogenous genotoxic stress downregulates the efficiency of transcription termination and reduces the efficiency of pre-mRNA cleavage at the polyadenylation (pA) sites. We report here that Mec1p impacts transcription termination at two distinct steps. Mec1p promotes recruitment of Pcf11p, a subunit of the cleavage factor IA (CF IA), to 3' ends

SUBMITTER: Mehta R 

PROVIDER: S-EPMC12508236 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature