Proteomic Profiling of Extracellular Vesicles Distinguishes Prostate Cancer Molecular Subtypes.

ABSTRACT: Prostate cancer is the most common non-cutaneous cancer among men in the United States. Most prostate cancers are driven by androgen receptor (AR) signalling, but there are an increasing number of cases that lose AR and gain neuroendocrine (NE) features (AR-/NE+) or lack both (AR-/NE-). These latter subtypes are particularly aggressive and lethal. Extracellular vesicles (EVs) have shown great potential as biomarkers for non-invasive liquid biopsy assays, as EVs contain biomolecules from their cells of origin. Here, we used a shotgun proteomics approach with mass spectrometry to interrogate the global proteome of EVs isolated from prostate cancer cell lines reflecting diverse clinical subtypes, including AR-/NE+ and AR-/NE- models. We identified 3952 EV proteins, which clustered largely by

SUBMITTER: Ludwig ML 

PROVIDER: S-EPMC12508259 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature