Improved method for elimination of bovine viral diarrhea virus from contaminated cell lines using virus inhibitor DB772.

ABSTRACT: Contamination of cell lines with bovine viral diarrhea virus (BVDV) derived from fetal bovine serum (FBS) is a major obstacle in cell biology experiments. Although the selection of BVDV-free FBS and constant viral checking are important to prevent the viral contamination of cells, they have been ineffective in the past contamination of several cell lines available from cell banks. Recently, a novel method to eliminate BVDV from one contaminated cell line, LFBK-α_vβ₆ cells, using BVDV inhibitor DB772 was reported. To confirm the reproducibility and possibility of practical use of this method in other cell lines, we attempted BVDV elimination using DB772 from five contaminated cell lines: BL3.1, EBTr, A26, FLK-BLV, and CC81. Treatment with DB772 significantly decreased v

SUBMITTER: Nishimori A 

PROVIDER: S-EPMC12508568 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature