Real-Time Monitoring of DNA Origami-Cell Interactions via Single Particle Tracking.

ABSTRACT: Due to the unique spatial addressability of DNA origami, targeting ligands can be specifically positioned onto the surface of the nanostructure, constituting an essential tool for studying ligand-receptor interactions at the cell surface. While the design and ligand incorporation into DNA origami nanostructures is well-established, the study of dynamic interactions with cell surfaces is still in the explorative phase, where an in-depth fundamental understanding of the molecular interaction dynamics remains underexplored. This study uniquely captures real-time encounters between DNA origami and cells in situ using single particle tracking (SPT). We functionalized DNA nanorods (NRs) with antibodies or aptamers specific to the epidermal growth factor receptor (EGFR) and used them to target EG

SUBMITTER: Van Zundert I 

PROVIDER: S-EPMC12508699 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature