Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides.

ABSTRACT: Flavonoids are valuable for pharmaceutical, cosmetic and food applications. However, poor solubility and bioavailability limit their widespread use. Biotechnological glycosylation of flavonoids helps address these limitations, but such bioprocesses remain constrained by the cost and availability of uridine diphosphate glucose (UDPG) and the inherent toxicity of flavonoids. In this study we demonstrate that Escherichia coli W is an optimal microbial host for glycosylation bioprocesses using sucrose as a carbon and UDPG source. Escherichia coli W outperforms the model E. coli K12 strain in terms of flavonoid tolerance and glycosylation capabilities. Optimization of sucrose metabolism through adaptive laboratory evolution (ALE) and targeted metabolic engineering to reroute glucose metabolism

SUBMITTER: Carranza-Saavedra D 

PROVIDER: S-EPMC12508785 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature