Integrative Spatial and Single-Nucleus Transcriptomics Elucidate Cell Lineage Dynamics in Human Tooth Morphogenesis.

ABSTRACT:

Introduction and aims

Understanding the identity of odontogenic cells is fundamental to advancing research in tooth development and regeneration. We aim to explore the cellular composition, differentiation pathways, and regulatory networks in human embryonic teeth during the transition from the cap stage to the early bell stage.

Methods

The spatial RNA sequencing and single-nucleus RNA sequencing to human embryonic tooth germs were obtained from four healthy aborted specimens and integrated with public database. The spatial and temporal gene expression patterns, cell distributions, and interactions in tooth germ and surrounding periodontal tissues spanning postconception weeks 12 to 18 were examined and validated by immunohistochemistry.

Results

Our analysis identifie

SUBMITTER: Chen X 

PROVIDER: S-EPMC12508835 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature