Development of PCR, qPCR and LAMP methods for the detection of <i>Spirometra mansoni</i> (Cestoda: Diphyllobothriidae) in the faeces of dogs and cats.

ABSTRACT: Spirometra mansoni is an important zoonotic parasitic tapeworm that is transmitted mainly through the faeces of definitive hosts such as cats and dogs. However, there is currently no molecular detection method for S. mansoni in the faeces of definitive hosts. Here, a PCR assay for S. mansoni in the faeces of definitive hosts was developed, and the effects of the sampling site, sample storage temperature and duration on the detection results were evaluated. qPCR assays and LAMP assays targeting the cytb gene were performed with optimized primers, probe concentrations and annealing temperatures. The sensitivity and specificity of three assays, namely, PCR, qPCR and LAMP, were evaluated. Applications in the field were conducted using these established assays. The s

SUBMITTER: Li W 

PROVIDER: S-EPMC12508883 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature