3D Printing of Chitosan Scaffolds and Films with Varying Roughness for Cultivation of Human Retinal Progenitor Cells.

ABSTRACT: Chitosan was used for three-dimensional (3D) printing of films and well-resolved scaffolds. Three different molecular weights with a comparable degree of de-N-acetylation were studied for 3D printing; inks were characterized using rheology, and the resulting two-dimensional (2D) and 3D architectures were characterized by scanning electron microscopy (SEM). Printing and fixation were optimized for retention of shape fidelity. The films and scaffolds were functionalized with a short peptide containing the integrin-binding arginylglycylaspartic acid (Arg-Gly-Asp, RGD) sequence using a postprinting grafting approach. The 2D films and 3D scaffolds prepared were studied as support materials for human retinal progenitor cells (hRPCs), and the properties of native and RGD-modified chitosan

SUBMITTER: Solberg A 

PROVIDER: S-EPMC12508935 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature