Modeling Ischemia-Reperfusion Injury in Stroke Using the BBB Chip.

ABSTRACT: Ischemic stroke is a leading cause of disability worldwide and poses a serious threat to public health, affecting tens of millions of people all over the world per year. The lack of a humanized organ model reflecting the real situation of patients has quite limited the pathogenesis research and therapeutic drug development of ischemic stroke. In this study, we developed an ischemic stroke model based on a high-throughput microfluidic chip device, simulating ischemia-reperfusion injury in an ischemic stroke. In this ischemic stroke model, we observed a series of injury characteristics, including significant blood-brain barrier (BBB) destruction, cell apoptosis, and mitochondrial dysfunction. Transcriptome sequencing analysis showed that the expression of genes involved in autophagy, oxidati

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12508954 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature