Ultrasound-assisted deep eutectic solvent extraction versus alkaline extraction: Functional and structural properties of hazelnut proteins.

ABSTRACT: This study compared conventional alkaline extraction and ultrasound-assisted deep eutectic solvent (DES-US) extraction for isolating functional proteins from hazelnut meal, an underutilized oilseed by-product. Alkaline extraction at pH 10 achieved the highest protein recovery (260 mg/g) and extraction efficiency (63 %), while DES-US systems yielded moderate soluble recoveries (∼200-220 mg/g) but showed reduced final protein yields (80 mg/g) due to precipitation challenges. Molecular weight profiles confirmed that both methods effectively preserved major storage proteins, including 7S and 11S globulins and 2S albumins. Secondary structural analyses revealed pronounced pH-induced denaturation in alkaline extracts, versus high α-helix retention in DES-US samples (e.g., DES-GLU 95.7 %, DES-SOR

SUBMITTER: Balballi EK 

PROVIDER: S-EPMC12509131 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature