Living bacteria-loaded antibacterial hydrogel-coated gauze: A revolutionary solution for multi-drug-resistant bacterial infections.

ABSTRACT: Chronic wounds infected by methicillin-resistant Staphylococcus aureus (MRSA) pose critical therapeutic challenges due to the prevalence of multi-drug-resistant (MDR) pathogens. To address this issue, a biocompatible live bacteria-loaded hydrogel-coated gauze (APAG) was developed by integrating an engineering Pseudomonas sp. SC11pLAFR-GFP with an alginate/ε-polylysine-polyacrylamide (Alg/ε-PL-PAAm) double-network hydrogel. The hierarchical hydrogel architecture, achieved through physical entrapment in the Alg/ε-PL core, ensures bacterial viability, while the chemically crosslinked PAAm outer layer regulates metabolite diffusion kinetics, synergistically enhancing therapeutic efficacy. The engineered bacteria hydrogel gauze (SC11@APAG) demonstrated sustained antimicrobi

SUBMITTER: Xie M 

PROVIDER: S-EPMC12509169 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature