Not Just Better Resolution: A Detailed Study of the Signal Distribution in Mid-Infrared Optical Photothermal Imaging.

ABSTRACT: The spatial resolution of an imaging system is generally evaluated by analysis of the point spread function (PSF), that is, the system response to a point source in the object plane. The PSF, albeit a fundamental concept in light microscopy, has been sparsely studied in optical photothermal infrared (O-PTIR) imaging. Although there is only a minor difference between the photothermal and optical intensity distributions in the radial direction, the axial photothermal signal is markedly different from the imaging response of conventional light microscopes. Previous studies of particles beyond the visible diffraction limit have shown that the O-PTIR signal has two lobes and strongly varies with the offset between the foci of the pump and the probe laser. We will therefore present theoretical a

SUBMITTER: Holub E 

PROVIDER: S-EPMC12509188 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature