Endoscopic indigo carmine spraying for evaluation of intestinal mucosal permeability: Prospective pilot study.

ABSTRACT:

Background and study aims

"Leaky gut," caused by increased mucosal permeability, plays a pivotal role in various diseases. However, few methods are available to evaluate intestinal mucosal permeability in the living human body. We established a novel method for evaluation of mucosal permeability using indigo carmine (IC).

Patients and methods

Subjects undergoing colonic endoscopy for screening of colon polyps or evaluation of ulcerative colitis (UC) severity were enrolled. IC was endoscopically sprayed in the cecum, and blood samples were obtained before spraying and at 30 and 60 minutes after. Serum IC level was analyzed by liquid chromatographer/mass spectrometer equipped with a Nexera HPLC system.

Results

In both the control (subjects screened for colon polyps) and

SUBMITTER: Fukui H 

PROVIDER: S-EPMC12509262 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature