Evaluation for CpG island methylation of FASN and SREBP promoter in Mongolian gerbil NAFLD model.

ABSTRACT:

Introduction

Methylation of the CpG island in the promoter and its nearby area is one of the most important ways to inhibit gene expression. The aim of this study was to examine the potential relationship between CpG island methylation for fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP-1).

Material and methods

Ten newborn gerbil pups formed the NB group, 10 adult gerbils with a normal diet formed the control group, 10 adult gerbils with a high-fat diet formed the nonalcoholic fatty liver disease (NAFLD) group, and 10 8-month-old gerbils formed the aged group. Blood and liver samples were collected for serum lipid detection and histopathology. A pyrosequencing technique was employed to determine the methylation rate. Then, the transcription

SUBMITTER: Wu J 

PROVIDER: S-EPMC12509570 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature