Mettl1-mediated m⁷G modification of Fgfr2 regulates osteogenic and chondrogenic differentiation of mesenchymal stem cells.

ABSTRACT: N7-methylguanosine (m⁷G) is a prevalent RNA modification and plays fundamental roles in embryonic stem cell self-renewal and differentiation. However, its specific contributions to mesenchymal stem cell differentiation during skeletal development remain poorly understood. In this study, we demonstrate that specific deletion of the m⁷G methyltransferase Mettl1 in mesenchymal lineage cells causes severe bone development defects, manifesting as dramatic limb shortening at birth. The absence of Mettl1 in mesenchymal stem cells significantly hinders osteoblast and chondrocyte differentiation. Integrative analyses of single-cell RNA-sequencing and m⁷G-MeRIP sequencing demonstrate that Mettl1 ablation disrupts m⁷G modifications of Fgfr2, resulting in re

SUBMITTER: Li Q 

PROVIDER: S-EPMC12509694 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature