Adductome-based identification of lysine monomethylation as a key post-translational protein modification in autoimmune diseases.

ABSTRACT: Post-translational modifications (PTMs) of proteins are efficient biological mechanisms for expanding the genetic code and for regulating cellular physiology. However, there have been no systematic approaches to profile all the PTMs critical for autoreactive neoantigen production or the etiology and pathology of autoimmune diseases. In the present study, to gain insight into protein PTMs associated with systemic lupus erythematosus (SLE), we applied a mass spectrometry-based method for the comprehensive analysis of modified amino acids ("adductome"). By comprehensively measuring modified lysines and histidines in mouse tissue homogenates from the control and SLE-prone MRL-lpr mice, we observed a significant decrease in lysine monomethylation as a unique alteration in the PTMs of SLE mice s

SUBMITTER: Yamaguchi K 

PROVIDER: S-EPMC12509770 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature