Fetal origin confers radioresistance on liver macrophages via p21^cip1/WAF1.

ABSTRACT:

Background & aims

Cells of hematopoietic origin, including macrophages, are generally radiation sensitive, but a subset of Kupffer cells (KCs) is relatively radioresistant. Here, we focused on the identity of the radioresistant KCs in unmanipulated mice and the mechanism of radioresistance.

Methods

We employed Emr1- and inducible CX3Cr1-based fate-mapping strategies combined with the RiboTag reporter to identify the total KCs and the embryo-derived KCs, respectively. The KC compartment was reconstituted with adult bone-marrow-derived KCs (bm-KCs) using clodronate depletion. Mice were lethally irradiated and transplanted with donor bone marrow, and the radioresistance of bone-marrow- or embryo-derived KCs was studied. Gene expression was analyzed using in situ mRNA isolation

SUBMITTER: Soysa R 

PROVIDER: S-EPMC12509797 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature